Admin: Created page with "=== Protocol === #Add 20 g of LB agar to a 1 L flash. #Fill flask to the 500 mL line with dH₂O. #Autoclave the flask. Note: have a water bath set to 50°C. #Whe..."

2021-02-01T19:37:30Z

Created page with "=== Protocol === #Add 20 g of LB agar to a 1 L flash. #Fill flask to the 500 mL line with dH2O. #Autoclave the flask. Note: have a water bath set to 50°C. #Whe..."

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=== Protocol ===

#Add 20 g of LB agar to a 1 L flash.
#Fill flask to the 500 mL line with dH2O.
#Autoclave the flask. Note: have a water bath set to 50°C.
#When the flask comes back from autoclaving, put it in the water bath to let it slowly cool down.
#Add 500 μL of antibiotic to the flask and swirl. 
#Light the bunsen burner, and add the LB agar to petri dishes. Add just enough of the mixture to cover the bottom of each petri dish.
#Let the plates cool down.
#For plates with ampicillin, add a blue line to the side of the plates, and for plates with kanamycin, add two black lines.
#Put plates back in the plastic bag and put them in the fridge.

Antibiotic Plates - Revision history

Admin: Created page with "=== Protocol === #Add 20 g of LB agar to a 1 L flash. #Fill flask to the 500 mL line with dH2O. #Autoclave the flask. Note: have a water bath set to 50°C. #Whe..."

Admin: Created page with "=== Protocol === #Add 20 g of LB agar to a 1 L flash. #Fill flask to the 500 mL line with dH₂O. #Autoclave the flask. Note: have a water bath set to 50°C. #Whe..."