Antibiotics - Revision history

Akipp4: /* Ampicillin */

2022-03-01T15:20:17Z

Ampicillin

← Older revision		Revision as of 15:20, 1 March 2022
Line 5:		Line 5:
	Preparation of 5 mL of 100 mg/mL:		Preparation of 5 mL of 100 mg/mL:

	#Add 0.5 g of ~~ampicilin~~ into a 12 mL tube.		#Add 0.5 g of ampicillin into a 12 mL tube.
	#Add 2.5 mL of ddH<sub>2</sub>O into the tube and mix gently by tapping the side of the tube.		#Add 2.5 mL of ddH<sub>2</sub>O into the tube and mix gently by tapping the side of the tube.
	#Add 2.5 mL of 100% ethanol and mix the tube again.		#Add 2.5 mL of 100% ethanol and mix the tube again.

Akipp4 at 18:25, 29 November 2021

2021-11-29T18:25:53Z

← Older revision		Revision as of 18:25, 29 November 2021
Line 8:		Line 8:
	#Add 2.5 mL of ddH<sub>2</sub>O into the tube and mix gently by tapping the side of the tube.		#Add 2.5 mL of ddH<sub>2</sub>O into the tube and mix gently by tapping the side of the tube.
	#Add 2.5 mL of 100% ethanol and mix the tube again.		#Add 2.5 mL of 100% ethanol and mix the tube again.
			#Filter with a 0.22 μm syringe filter
	#Aliquot out 1 mL into each of 5 microcentrifuge tubes.		#Aliquot out 1 mL into each of 5 microcentrifuge tubes.
	#Label the tubes as 1000x ampicillin and write the date.~~<br>~~		#Label the tubes as 1000x ampicillin and write the date.
	#Place tubes in the antibiotics box in the -20 °C freezer.<br>		#Place tubes in the antibiotics box in the -20 °C freezer.<br>

Admin: Created page with "== Protocol == ==== Ampicillin
==== Preparation of 5 mL of 100 mg/mL: #Add 0.5 g of ampicilin into a 12 mL tube. #Add 2.5 mL of ddH₂O into the tube and mix g..."

2021-02-01T19:37:16Z

Created page with "== Protocol == ==== Ampicillin ==== Preparation of 5 mL of 100 mg/mL: #Add 0.5 g of ampicilin into a 12 mL tube. #Add 2.5 mL of ddH2O into the tube and mix g..."

New page

== Protocol ==

==== Ampicillin ====

Preparation of 5 mL of 100 mg/mL:

#Add 0.5 g of ampicilin into a 12 mL tube.
#Add 2.5 mL of ddH2O into the tube and mix gently by tapping the side of the tube.
#Add 2.5 mL of 100% ethanol and mix the tube again.
#Aliquot out 1 mL into each of 5 microcentrifuge tubes.
#Label the tubes as 1000x ampicillin and write the date. 
#Place tubes in the antibiotics box in the -20 °C freezer. 

Note: Ampicillin stock will only be good for approximately 6 months. 

==== Kanamycin ====

Preparation of 5 mL of 50 mg/mL 

#Add 0.25 g of kanamycin into a 12 mL tube. 
#Add 5 mL ddH2O into the tube and mix by shaking tube. 
#Aliquot out 1 mL into each of 5 microcentrifuge tubes. 
#Label the tubes as 1000x kanamycin and write the date. 
#Place the tubes in the antibiotics box in the -20 °C freezer. 

Note: Kanamycin tubes must be kept in the fridge after being removed from the freezer.

Antibiotics - Revision history

Akipp4: /* Ampicillin */

Akipp4 at 18:25, 29 November 2021

Admin: Created page with "== Protocol == ==== Ampicillin ==== Preparation of 5 mL of 100 mg/mL: #Add 0.5 g of ampicilin into a 12 mL tube. #Add 2.5 mL of ddH2O into the tube and mix g..."

Admin: Created page with "== Protocol == ==== Ampicillin
==== Preparation of 5 mL of 100 mg/mL: #Add 0.5 g of ampicilin into a 12 mL tube. #Add 2.5 mL of ddH₂O into the tube and mix g..."