Admin: Created page with "=== LB Broth
=== To 200 mL ddH₂O add:
*Pre-made: **6.25g Miller LB powder
*'''''OR'''''
2.5g Tryptone
2.5g NaCl
**1.25g Yeast extract..."

2021-02-01T19:25:31Z

Created page with "=== LB Broth === To 200 mL ddH2O add: *Pre-made: **6.25g Miller LB powder *'''''OR''''' **2.5g Tryptone **2.5g NaCl **1.25g Yeast extract..."

New page

=== LB Broth ===

To 200 mL ddH2O add: 
*Pre-made:
**6.25g Miller LB powder 
*'''''OR''''' 
**2.5g Tryptone 
**2.5g NaCl 
**1.25g Yeast extract 
**pH to 7.5 if necessary
 
*Bring to 250 mL volume 

Aliquot into bottles (~50 mL per bottle) for autoclaving. The bottle caps should be held very loosely, covered in foil and labelled. The bottles should be placed in a steel basket.

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=== SOC Medium ===
''Used to improve yield following transformation, during recovery phase.''

To 35 mL ddH2O add: 

*0.375g Yeast Extract 
*1.5g Tryptone 
*0.045g NaCl 
*0.09g MgSO4 
*0.014g KCl 

Bring to 75 mL volume and autoclave. 

Then add: 
*0.75mL 1M MgCl2 
*0.24g Glucose
'''Finally, sterilize the media by passing it through a 0.2μm filter'''

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=== Terrific Broth (TB) ===
''Used to grow E. coli ZYCY10P3S2T during minicircle generation protocol ''

To 150 mL ddH2O in a 500 mL bottle, add: 
*4.8g Yeast Extract 
*4g Tryptone 
*4mL Glycerol 
Bring to volume of 180 mL and autoclave 
To 20 mL ddH2O, add: 
*0.046g KH2PO4 (potassium phosphate monobasic) 
*0.25g K2HPO4 (potassium phosphate dibasic) 
Filter sterilize and add to the autoclaved portion, for final volume of 200 mL

Bacterial Growth Media - Revision history

Admin: Created page with "=== LB Broth === To 200 mL ddH2O add: *Pre-made: **6.25g Miller LB powder *'''''OR''''' **2.5g Tryptone **2.5g NaCl **1.25g Yeast extract..."

Admin: Created page with "=== LB Broth
=== To 200 mL ddH₂O add:
*Pre-made: **6.25g Miller LB powder
*'''''OR'''''
2.5g Tryptone
2.5g NaCl
**1.25g Yeast extract..."