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= Maintenance of Common Cell Lines =

{| width="95%" border="1" cellpadding="1" cellspacing="1"
|-
| '''Cell Type'''
| '''Media'''
| '''Maintenance'''
| '''Splitting'''
| '''Notes'''
|-
| RAW267.4 (Murine macropahge)
| DMEM + 10% FBS
| Grow to 80% confluency, split 1:5 to 1:10
| Scrape
| Prone to genetic drift; use for 20 passages or less
|-
| J774.1 (Murine macropahge)
| DMEM + 10% FBS
| Grow to 80% confluency, split 1:5 to 1:10
| Scrape
|
|-
| THP-1 (Human monocytic)
| RPMI 1640 + 10% FBS
| Spin to recover, split no ore than 1:15
| Suspension
| 0.05 mM 2-mercaptoethanol can be added to enhance growth
|-
| CHO-K1 (Hampster epithelial)
| ''Preferred'': Hams F-12 + 10% FBS ''Alternative'': DMEM + 10% FBS
| Grow to 80% confluency, split 1:5 to 1:10
| Trypsin-EDTA
| Requires proline in media, therefore RPMI should not be used
|-
| HeLa (Human epithelial)
| ''Preferred'': DMEM + 5-10% FBS ''Alternative'': EMEM + 5-10% FBS
| Grow to 80% confluency, split 1:5 to 1:10
| Trypsin-EDTA
|
|-
| HEK (Human epithelial)
| DMEM + 10% FBS
| Grow to 80% confluency, split 1:5 to 1:10
| Trypsin-EDTA
|
|-
| 12CA5 (Murine hybridoma)
| ''Growth'': DMEM or RPMI + 10% HI-FBS ''Antibody Produciton'': Hybridoma SFM
| Grow to high denisty in DMEM; spin to concentrate and resuspend in SFM for antibody production 
| Suspension
| Supplement growth media with non-essential amino acids, L-glutamine, sodium pyruvate and MEM Vitamins if producing antibody in RMPI or DMEM instead of SFM. 
|-
| COS-7 (Monkey epithelial)
| DMEM + 10% FBS
| Grow to 80% confluency, split 1:5 to 1:10
| Trypsin-EDTA
|
|-
| HEK293T (Human epithelial)
| DMEM + 10% FBS
| Grow to 80% confluency, split 1:5 to 1:10
| Trypsin-EDTA
|
|-
| Jurkat (Human T cell)
| RPMI + 10% FBS
| Grow to 80% confluency, split 1:5 to 1:10
| Trypsin-EDTA
|
|-
| C57 (Immortalised peritoneal macrophage from C57/Bl6 mouse)
| RPMI + 10% FBS
| Grow to 80% confluency, then split 1:5 to 1:10
| Scrape
|
|}

=== Notes: ===

*FBS = fetal bovine serum (AKA FCS)
*HI-FBS = heat inactivated FBS. Heat-inactivate by heating 50 mL of FBS to 55C for 30 min.
*Antibiotics/mycotics can be added to most cultures, but shoudl be removed for functional assays.

Cell Culture Guidelines - Revision history

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