Admin: Created page with "= Protocol = #Cool cells if needed, protocol also works at 37C #Wash 2X with PBS at required temperature #Cover cells with PEG media, incubate 10-20 min #Wash carefully wi..."

2021-02-01T19:56:31Z

Created page with "= Protocol = #Cool cells if needed, protocol also works at 37C #Wash 2X with PBS at required temperature #Cover cells with PEG media, incubate 10-20 min #Wash carefully wi..."

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= Protocol =

#Cool cells if needed, protocol also works at 37C
#Wash 2X with PBS at required temperature
#Cover cells with PEG media, incubate 10-20 min
#Wash carefully with PBS to remove PEG.<br>

= Recipes =

===== 1L PEG Media: =====

*4.5g Sucrose
*1.907g 5mM EGTA
*1L PBS

===== 10ml PEG Media: =====

*0.045g Sucrose
*0.019g 5mM EGTA
*10ml PBS
<pre>Warning: cells will be weakly adherent after this procedure; take care to minimize shear in order to avoid loosing the cells.
</pre>

Inhibition of Focal Contact Signaling - Revision history

Admin: Created page with "= Protocol = #Cool cells if needed, protocol also works at 37C #Wash 2X with PBS at required temperature #Cover cells with PEG media, incubate 10-20 min #Wash carefully wi..."