Admin: Created page with "Synchronised phagocytosis is used to ensure that all observed phagocytosis events in a maple have occurred after a similar amount of time. This is useful for microscopy..."

2021-02-01T19:38:43Z

Created page with "Synchronised phagocytosis is used to ensure that all observed phagocytosis events in a maple have occurred after a similar amount of time. This is useful for microscopy..."

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Synchronised phagocytosis is used to ensure that all observed phagocytosis events in a maple have occurred after a similar amount of time.  This is useful for microscopy and molecular biology, when discrete stages of phagocytosis want to be investigated.

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#Prepare phagocytes and target particles as per usual.
#Remove phagocytes from incubator and allow to cool to room temperature.
#Add the desired number of target particles, and centrifuge sample for 1 min at 1500 RPM (~500xG).
#Let sit at room temperature for 15-20 min.  During this time phagocytes will bind particles, but not ingest them.
#Wash phagocytes 2-3X with PBS or media to remove unbound target particles.
#Activate phagocytosis by swapping media for media warmed to 37<sup>o</sup>C, then transfer to an incubator for the desired amount of time.
#Terminate phagocytosis by transferring cells to ice, adding a fixative, or lysing cells.

Synchronised Phagocytosis - Revision history

Admin: Created page with "Synchronised phagocytosis is used to ensure that all observed phagocytosis events in a maple have occurred after a similar amount of time. This is useful for microscopy..."