MediaWiki API result
This is the HTML representation of the JSON format. HTML is good for debugging, but is unsuitable for application use.
Specify the format parameter to change the output format. To see the non-HTML representation of the JSON format, set format=json.
See the complete documentation, or the API help for more information.
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"comment": "Created page with \" Protocol 1. Check the NEB (or other supplier) website to confirm enzyme compatibility 2. In a PCR tube add the reaction components according to the table listed below 3. Incubate at 37\u00b0C for 30 min if time-saver qualified, 60 minutes if not Example Reaction\t Component\tVolume ddH2O\tUp to 50 \u00b5L 10X rCutSmart Buffer\t5 \u00b5L DNA\tx (1 \u00b5g) Enzyme 1\t1 \u00b5L Enzyme 2\t1 \u00b5L Notes -\tNEB High-Fidelity (HF) enzymes are ordered whenever possible, they all use rCutSmart reactio...\""
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"comment": "Created page with \"==== Before the Experiment ==== # Prepare substrates and place them into the wells of a 12-well plate. These can be prepared by coating slides with a ligand of interest, or by [[Micropatterning Proteins|microprinting]]. # Transfect cells if required, as per usual protocols. # Split macrophages into wells without coverslips, 1 well/frustrated phagocytosis assay (~250,000 cells/well). ==== Experiment: Fixed-Cell ==== # Pre-warm the substrates and centrifuges to 37C. # I...\""
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"comment": "Created page with \"This protocol is based on IgG micropatterning methods as published by Freeman ''et al''. <ref>Freeman et al., (2016) \u201cIntegrins Form an Expanding Diffusional Barrier That Coordinates Phagocytosis.\u201d Cell. V164#1, pp 128-40. http://linkinghub.elsevier.com/retrieve/pii/S0092867415015676</ref>It may need to be optimized for other proteins. ==== Preparation of the Microprinting Stamp. ==== This is optimized for a PDMS based stamp and may have to be modified for stamps m...\""
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