Antibiotics

Protocol

Ampicillin

Preparation of 5 mL of 100 mg/mL:

1. Add 0.5 g of ampicillin into a 12 mL tube.
2. Add 2.5 mL of ddH₂O into the tube and mix gently by tapping the side of the tube.
3. Add 2.5 mL of 100% ethanol and mix the tube again.
4. Filter with a 0.22 μm syringe filter
5. Aliquot out 1 mL into each of 5 microcentrifuge tubes.
6. Label the tubes as 1000x ampicillin and write the date.
7. Place tubes in the antibiotics box in the -20 °C freezer.
   

Note: Ampicillin stock will only be good for approximately 6 months.

Kanamycin

Preparation of 5 mL of 50 mg/mL

1. Add 0.25 g of kanamycin into a 12 mL tube.
   
2. Add 5 mL ddH₂O into the tube and mix by shaking tube.
   
3. Aliquot out 1 mL into each of 5 microcentrifuge tubes.
   
4. Label the tubes as 1000x kanamycin and write the date.
   
5. Place the tubes in the antibiotics box in the -20 °C freezer.
   

Note: Kanamycin tubes must be kept in the fridge after being removed from the freezer.