https://wiki.phagocytes.ca/index.php?action=history&feed=atom&title=Water_Bath_Antibiotic_SolutionWater Bath Antibiotic Solution - Revision history2026-09-21T17:21:28ZRevision history for this page on the wikiMediaWiki 1.40.1https://wiki.phagocytes.ca/index.php?title=Water_Bath_Antibiotic_Solution&diff=23&oldid=prevAdmin: Created page with "= Reagents Needed = *Copper Sulfate (Cu<sub>2</sub>SO<sub>4</sub>) *EDTA *Dish soap or other detergent (i.e. Tween/Triton) = Preparation = Fill water bath with tap water (typ..."2021-02-01T19:37:53Z<p>Created page with "= Reagents Needed = *Copper Sulfate (Cu<sub>2</sub>SO<sub>4</sub>) *EDTA *Dish soap or other detergent (i.e. Tween/Triton) = Preparation = Fill water bath with tap water (typ..."</p>
<p><b>New page</b></p><div>= Reagents Needed =<br />
*Copper Sulfate (Cu<sub>2</sub>SO<sub>4</sub>)<br />
*EDTA<br />
*Dish soap or other detergent (i.e. Tween/Triton)<br />
<br />
= Preparation =<br />
Fill water bath with tap water (typically 6 L is needed). Weigh out Cu<sub>2</sub>SO<sub>4</sub> necessary to prepare 1 g/L final concentration (6 g for 6 L). Pre-dissolve the Cu<sub>2</sub>SO<sub>4</sub> in a small beaker of water, then mix into water bath. Add 1:9500 of 0.5 M EDTA (650 uL into 6 L). Add a very small amount of dish soap or 100-1000 uL of another detergent.<br />
<br />
<pre>Copper sulfate is a natural antibiotic compound. EDTA chelates the essential ions needed for bacterial growth. The detergent works as a surfactant to any clumping</pre></div>Admin