Bradford Assay: Difference between revisions
Jump to navigation
Jump to search
(The Bradford assay is used to determine the concentration of total protein within a sample relative to a standard curve.) |
(Started a new page for Bradford Assay - will finish when optimized) |
||
| Line 1: | Line 1: | ||
Bradford | '''Introduction''' | ||
The Bradford assay should be used to quantify total protein within a sample relative to a standard curve. This is important when running proteins on SDS-PAGE/Western blot, where each lane must contain an identical amount of total protein. This way, the intensity of the bands can be compared between different lanes, as standardizing the amount of protein loaded per lane will account for any variation in cell count from which the proteins were derived. | |||
'''Protocol''' | |||
# | |||
Revision as of 15:57, 13 January 2022
Introduction
The Bradford assay should be used to quantify total protein within a sample relative to a standard curve. This is important when running proteins on SDS-PAGE/Western blot, where each lane must contain an identical amount of total protein. This way, the intensity of the bands can be compared between different lanes, as standardizing the amount of protein loaded per lane will account for any variation in cell count from which the proteins were derived.
Protocol