Antibiotics

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Protocol

Ampicillin

Preparation of 5 mL of 100 mg/mL:

  1. Add 0.5 g of ampicilin into a 12 mL tube.
  2. Add 2.5 mL of ddH2O into the tube and mix gently by tapping the side of the tube.
  3. Add 2.5 mL of 100% ethanol and mix the tube again.
  4. Aliquot out 1 mL into each of 5 microcentrifuge tubes.
  5. Label the tubes as 1000x ampicillin and write the date.
  6. Place tubes in the antibiotics box in the -20 °C freezer.

Note: Ampicillin stock will only be good for approximately 6 months.

Kanamycin

Preparation of 5 mL of 50 mg/mL

  1. Add 0.25 g of kanamycin into a 12 mL tube.
  2. Add 5 mL ddH2O into the tube and mix by shaking tube.
  3. Aliquot out 1 mL into each of 5 microcentrifuge tubes.
  4. Label the tubes as 1000x kanamycin and write the date.
  5. Place the tubes in the antibiotics box in the -20 °C freezer.

Note: Kanamycin tubes must be kept in the fridge after being removed from the freezer.


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