Inhibition of Focal Contact Signaling
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- Cool cells if needed, protocol also works at 37C
- Wash 2X with PBS at required temperature
- Cover cells with PEG media, incubate 10-20 min
- Wash carefully with PBS to remove PEG.
1L PEG Media:
- 4.5g Sucrose
- 1.907g 5mM EGTA
- 1L PBS
10ml PEG Media:
- 0.045g Sucrose
- 0.019g 5mM EGTA
- 10ml PBS
Warning: cells will be weakly adherent after this procedure; take care to minimize shear in order to avoid loosing the cells.