User contributions for Admin

17 September 2024

• 11:3711:37, 17 September 2024 diff hist +47‎ Immunostaining ‎ →‎PEM Buffer: current Tag: Visual edit

21 August 2024

• 17:4917:49, 21 August 2024 diff hist 0‎ THP1 Culture and Differentiation ‎ →‎Protocol current Tag: Visual edit
• 14:5814:58, 21 August 2024 diff hist +6‎ m THP1 Culture and Differentiation ‎ →‎Subtype-Specific Macrophage Differentiation Tag: Visual edit

7 August 2024

• 15:1315:13, 7 August 2024 diff hist +1,092‎ THP1 Culture and Differentiation ‎No edit summary Tag: Visual edit
• 12:4012:40, 7 August 2024 diff hist +135‎ m Staining for GSD ‎ →‎GSD Buffer 4 (AlexaFluor Optimized Buffer): current Tag: Visual edit
• 12:3212:32, 7 August 2024 diff hist +9‎ Staining for GSD ‎ →‎GSD Imaging Buffers Tag: Visual edit: Switched
• 12:3112:31, 7 August 2024 diff hist +633‎ m Staining for GSD ‎ →‎Recipes Tag: Visual edit: Switched

29 July 2024

• 19:0419:04, 29 July 2024 diff hist +33‎ THP1 Culture and Differentiation ‎No edit summary Tag: Visual edit
• 19:0419:04, 29 July 2024 diff hist +14‎ THP1 Culture and Differentiation ‎No edit summary Tag: Visual edit
• 19:0219:02, 29 July 2024 diff hist +1,944‎ N THP1 Culture and Differentiation ‎ Created page with "==== Maintenance ==== THP1 cells ([https://www.atcc.org/products/tib-202?matchtype=&network=x&device=c&adposition=&keyword=&gad_source=1&gclid=Cj0KCQjw-5y1BhC-ARIsAAM_oKlZTKs5zplX22cd1pVZB7E0-Cq7fDL5pcruuijrK0PqV9a-5LG5l24aAoEqEALw_wcB ATCC TIB-202]) should be maintained in RPMI + 10% FBS, with cells split 1:5 into fresh medium when the cell density reaches 1 x 10⁶ cells/mL. Do not allow cell density to exceed 1.5 x 10⁶ cells/mL. '''Classical Mac..." Tag: Visual edit
• 18:5118:51, 29 July 2024 diff hist +39‎ m Main Page ‎No edit summary Tag: Visual edit

15 July 2024

• 15:2915:29, 15 July 2024 diff hist +1‎ m Staining for GSD ‎ →‎GSD Imaging Buffers Tag: Visual edit
• 14:3914:39, 15 July 2024 diff hist −2‎ m Staining for GSD ‎No edit summary Tag: Visual edit
• 14:3814:38, 15 July 2024 diff hist +209‎ m Staining for GSD ‎No edit summary Tag: Visual edit

10 July 2024

• 15:1215:12, 10 July 2024 diff hist +1,756‎ N Frustrated Phagocytosis ‎ Created page with "==== Before the Experiment ==== # Prepare substrates and place them into the wells of a 12-well plate. These can be prepared by coating slides with a ligand of interest, or by microprinting. # Transfect cells if required, as per usual protocols. # Split macrophages into wells without coverslips, 1 well/frustrated phagocytosis assay (~250,000 cells/well). ==== Experiment: Fixed-Cell ==== # Pre-warm the substrates and centrifuges to 37C. # I..." current Tag: Visual edit
• 14:3814:38, 10 July 2024 diff hist +29‎ m Main Page ‎ →‎Index of Protocols Tag: Visual edit

2 July 2024

• 16:0316:03, 2 July 2024 diff hist 0‎ m Micropatterning Proteins ‎ →‎Patterning a coverslip. current Tag: Visual edit

19 June 2024

• 16:4416:44, 19 June 2024 diff hist +21‎ m Micropatterning Proteins ‎No edit summary Tag: Visual edit
• 16:4316:43, 19 June 2024 diff hist +2,201‎ N Micropatterning Proteins ‎ Created page with "This protocol is based on IgG micropatterning methods as published by Freeman ''et al''. <ref>Freeman et al., (2016) “Integrins Form an Expanding Diffusional Barrier That Coordinates Phagocytosis.” Cell. V164#1, pp 128-40. http://linkinghub.elsevier.com/retrieve/pii/S0092867415015676</ref>It may need to be optimized for other proteins. ==== Preparation of the Microprinting Stamp. ==== This is optimized for a PDMS based stamp and may have to be modified for stamps m..." Tag: Visual edit
• 16:2916:29, 19 June 2024 diff hist +28‎ m Main Page ‎ →‎Index of Protocols Tag: Visual edit

7 June 2024

• 17:1417:14, 7 June 2024 diff hist +718‎ Staining for GSD ‎No edit summary Tag: Visual edit

18 May 2023

• 13:3113:31, 18 May 2023 diff hist +3,266‎ Preparation of Digestion-Tracking Bacteria ‎ New protocol with new bacteria and known OD600s current Tag: Visual edit

23 February 2023

• 16:5916:59, 23 February 2023 diff hist −117‎ 3D Printed Microscopy Chambers ‎ →‎Chamber Assembly current Tag: Visual edit

31 January 2023

• 14:5914:59, 31 January 2023 diff hist −3‎ m Primary Macrophage Culture ‎No edit summary current Tag: Visual edit
• 14:5914:59, 31 January 2023 diff hist −5‎ m Primary Macrophage Culture ‎ →‎Culture of Bone Marrow Cells: Tag: Visual edit

17 November 2022

• 18:4118:41, 17 November 2022 diff hist +20‎ m Acid Washing Coverslips ‎No edit summary current Tag: Visual edit

3 March 2022

• 18:3118:31, 3 March 2022 diff hist +259‎ m 3D Printed Microscopy Chambers ‎No edit summary Tag: Visual edit
• 16:2616:26, 3 March 2022 diff hist +68‎ m 3D Printed Microscopy Chambers ‎No edit summary Tag: Visual edit
• 15:4415:44, 3 March 2022 diff hist +152‎ m 3D Printed Microscopy Chambers ‎ →‎DLP Printing Tag: Visual edit
• 15:4215:42, 3 March 2022 diff hist +89‎ m 3D Printed Microscopy Chambers ‎ →‎FDM (Filament) Printing Tag: Visual edit
• 15:3915:39, 3 March 2022 diff hist +1,405‎ m 3D Printed Microscopy Chambers ‎No edit summary Tag: Visual edit
• 15:3615:36, 3 March 2022 diff hist +10‎ N File:MagnetTool.png ‎No edit summary current
• 15:3515:35, 3 March 2022 diff hist +14‎ N File:50mm BasePlate.png ‎No edit summary current
• 15:3515:35, 3 March 2022 diff hist +14‎ N File:18mm BasePlate.png ‎No edit summary current

2 March 2022

• 19:0319:03, 2 March 2022 diff hist 0‎ m 3D Printed Microscopy Chambers ‎ →‎DLP Printing Tag: Visual edit
• 19:0219:02, 2 March 2022 diff hist −39‎ m 3D Printed Microscopy Chambers ‎ →‎DLP Printing Tag: Visual edit
• 19:0119:01, 2 March 2022 diff hist +17‎ m 3D Printed Microscopy Chambers ‎ →‎FDM (Filament) Printing Tag: Visual edit
• 19:0119:01, 2 March 2022 diff hist 0‎ m 3D Printed Microscopy Chambers ‎No edit summary Tag: Visual edit
• 19:0019:00, 2 March 2022 diff hist +4,040‎ 3D Printed Microscopy Chambers ‎No edit summary Tag: Visual edit
• 18:4218:42, 2 March 2022 diff hist +2,437‎ 3D Printed Microscopy Chambers ‎No edit summary Tag: Visual edit
• 18:0318:03, 2 March 2022 diff hist +3,325‎ 3D Printed Microscopy Chambers ‎No edit summary Tag: Visual edit
• 18:0118:01, 2 March 2022 diff hist +10‎ N File:ResinPrint.png ‎No edit summary current
• 17:5417:54, 2 March 2022 diff hist +5‎ N File:Resin.png ‎No edit summary current
• 17:4617:46, 2 March 2022 diff hist +8‎ N File:Assembly.png ‎No edit summary current
• 17:3717:37, 2 March 2022 diff hist +190‎ m 3D Printed Microscopy Chambers ‎No edit summary Tag: Visual edit
• 17:3617:36, 2 March 2022 diff hist +14‎ N File:18mm Lid Clear.png ‎No edit summary current
• 17:3617:36, 2 March 2022 diff hist +10‎ N File:18mm Lid Solid.png ‎No edit summary current
• 17:3617:36, 2 March 2022 diff hist +10‎ N File:18mm Align.png ‎No edit summary current
• 17:3517:35, 2 March 2022 diff hist +14‎ N File:50mm Lid Clear.png ‎No edit summary current
• 17:3517:35, 2 March 2022 diff hist +14‎ N File:50mm Lid Solid.png ‎No edit summary current