Restriction Digest: Revision history

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17 January 2025

  • curprev 18:5618:56, 17 January 2025‎ Akipp4 talk contribs‎ 968 bytes +150‎ No edit summary Tag: Visual edit
  • curprev 18:5218:52, 17 January 2025‎ Akipp4 talk contribs‎ 818 bytes +818‎ Created page with " Protocol 1. Check the NEB (or other supplier) website to confirm enzyme compatibility 2. In a PCR tube add the reaction components according to the table listed below 3. Incubate at 37°C for 30 min if time-saver qualified, 60 minutes if not Example Reaction Component Volume ddH2O Up to 50 µL 10X rCutSmart Buffer 5 µL DNA x (1 µg) Enzyme 1 1 µL Enzyme 2 1 µL Notes - NEB High-Fidelity (HF) enzymes are ordered whenever possible, they all use rCutSmart reactio..."
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