User contributions for Akipp4

17 January 2025

• 19:5519:55, 17 January 2025 diff hist +344‎ Gibson Assembly ‎No edit summary current Tag: Visual edit
• 19:0619:06, 17 January 2025 diff hist −1,502‎ Gibson Assembly ‎ →‎Materials Tag: Visual edit
• 18:5618:56, 17 January 2025 diff hist +150‎ Restriction Digest ‎No edit summary current Tag: Visual edit
• 18:5218:52, 17 January 2025 diff hist +818‎ N Restriction Digest ‎ Created page with " Protocol 1. Check the NEB (or other supplier) website to confirm enzyme compatibility 2. In a PCR tube add the reaction components according to the table listed below 3. Incubate at 37°C for 30 min if time-saver qualified, 60 minutes if not Example Reaction Component Volume ddH2O Up to 50 µL 10X rCutSmart Buffer 5 µL DNA x (1 µg) Enzyme 1 1 µL Enzyme 2 1 µL Notes - NEB High-Fidelity (HF) enzymes are ordered whenever possible, they all use rCutSmart reactio..."
• 18:5018:50, 17 January 2025 diff hist +12‎ Main Page ‎ →‎DNA/Cloning current
• 18:5018:50, 17 January 2025 diff hist −21‎ Main Page ‎ →‎DNA/Cloning
• 18:4918:49, 17 January 2025 diff hist +8‎ Main Page ‎No edit summary Tag: Visual edit
• 18:4818:48, 17 January 2025 diff hist −3‎ Digests ‎ Undo revision 244 by Akipp4 (talk) current Tag: Undo
• 18:4518:45, 17 January 2025 diff hist −1‎ Main Page ‎ →‎DNA/Cloning
• 18:4318:43, 17 January 2025 diff hist 0‎ Main Page ‎ →‎DNA/Cloning
• 18:4318:43, 17 January 2025 diff hist +14‎ Main Page ‎ →‎DNA/Cloning
• 18:4218:42, 17 January 2025 diff hist +3‎ Digests ‎ updated protocoll to reflect neb enzymes Tag: Visual edit
• 18:4018:40, 17 January 2025 diff hist −665‎ Digests ‎ →‎Protocol Tag: Visual edit

2 August 2024

• 16:5416:54, 2 August 2024 diff hist +1‎ Coomassie Staining ‎No edit summary current Tag: Visual edit

1 March 2022

• 15:2015:20, 1 March 2022 diff hist +1‎ m Antibiotics ‎ →‎Ampicillin current Tag: Visual edit

25 February 2022

• 15:1515:15, 25 February 2022 diff hist −245‎ Agarose Gels ‎ →‎For a 0.7% gel: current Tag: Visual edit
• 15:0715:07, 25 February 2022 diff hist +172‎ Agarose Gels ‎ Undo revision 160 by Akipp4 (talk) Tag: Undo
• 15:0515:05, 25 February 2022 diff hist −172‎ Agarose Gels ‎ →‎For a 0.7% gel: Tag: Visual edit
• 15:0415:04, 25 February 2022 diff hist 0‎ Main Page ‎No edit summary Tag: Visual edit

23 February 2022

• 20:2520:25, 23 February 2022 diff hist −141‎ m Main Page ‎No edit summary Tag: Visual edit
• 20:1620:16, 23 February 2022 diff hist −11‎ Main Page ‎ →‎Index of Protocols Tag: Visual edit
• 20:1520:15, 23 February 2022 diff hist +17‎ Main Page ‎ →‎Index of Protocols Tag: Visual edit
• 20:1420:14, 23 February 2022 diff hist −1‎ Main Page ‎ →‎Index of Protocols Tag: Visual edit
• 20:1220:12, 23 February 2022 diff hist −11‎ Main Page ‎ →‎Index of Protocols Tag: Visual edit
• 20:1020:10, 23 February 2022 diff hist +164‎ Main Page ‎No edit summary Tag: Visual edit

18 January 2022

• 19:2919:29, 18 January 2022 diff hist −35‎ Bradford Assay ‎No edit summary Tag: Visual edit
• 14:3214:32, 18 January 2022 diff hist −1‎ Main Page ‎No edit summary Tag: Visual edit
• 14:3014:30, 18 January 2022 diff hist 0‎ Main Page ‎No edit summary Tag: Visual edit

17 January 2022

• 17:3717:37, 17 January 2022 diff hist −19‎ Entrance Protocol ‎No edit summary current Tag: Visual edit
• 17:3217:32, 17 January 2022 diff hist +3‎ Exit Protocol ‎No edit summary current Tag: Visual edit
• 16:2616:26, 17 January 2022 diff hist +26‎ Main Page ‎No edit summary Tag: Visual edit
• 16:2016:20, 17 January 2022 diff hist +49‎ Main Page ‎No edit summary Tag: Visual edit

11 January 2022

• 17:1717:17, 11 January 2022 diff hist 0‎ Main Page ‎No edit summary Tag: Visual edit
• 17:1617:16, 11 January 2022 diff hist +1,238‎ N Transduction of THP-1s ‎ Created page with "'''Transduction Reaction''' * The volume of the reaction is based on the amount of virus available, the number of cells and the MOI being used   *   Prepare the mixture as follows: **  X uL of concentrated virus **  X uL of SF Macrophage Media (chose this volume based on the vessel the cells will be grown in ** 1000X polybrene '''Protocol''' # Count cells, add cells to appropriate size tube, spin down at 400 x G for 5 minutes # Re-suspend cells in the transduct..." current Tag: Visual edit
• 17:0917:09, 11 January 2022 diff hist −28‎ Main Page ‎No edit summary Tag: Visual edit
• 17:0117:01, 11 January 2022 diff hist +41‎ Main Page ‎ →‎Index of Protocols Tag: Visual edit

6 December 2021

• 14:5414:54, 6 December 2021 diff hist +8‎ Titering Pseudo-typed Lentiviruses ‎No edit summary current Tag: Visual edit
• 14:5414:54, 6 December 2021 diff hist +2‎ Titering Pseudo-typed Lentiviruses ‎No edit summary Tag: Visual edit
• 14:5114:51, 6 December 2021 diff hist 0‎ Titering Pseudo-typed Lentiviruses ‎No edit summary Tag: Visual edit
• 14:2514:25, 6 December 2021 diff hist −5‎ Titering Pseudo-typed Lentiviruses ‎No edit summary Tag: Visual edit
• 14:2514:25, 6 December 2021 diff hist +107‎ Titering Pseudo-typed Lentiviruses ‎No edit summary Tag: Visual edit

29 November 2021

• 19:2219:22, 29 November 2021 diff hist 0‎ Titering Pseudo-typed Lentiviruses ‎No edit summary Tag: Visual edit
• 18:2518:25, 29 November 2021 diff hist +35‎ m Antibiotics ‎No edit summary Tag: Visual edit

25 November 2021

• 15:1915:19, 25 November 2021 diff hist +40‎ Main Page ‎No edit summary Tag: Visual edit
• 15:1715:17, 25 November 2021 diff hist +3,043‎ N Titering Pseudo-typed Lentiviruses ‎ Created page with "-       for titering one “batch” of collected virus '''Cell Seeding''' 1.     Seed 6 wells of a 12 well plate with 30 000 – 50 000 HeLas per well on coverslips '''Transduction''' 1.     Prepare “transduction media” by mixing 6 µL of polybrene (1000X) in 6 mL of DMEM+10% FBS 2.     Remove media from the plate and replace with 900 µL of transduction media in each well 3.     Begin the serial dilution by adding 100 µL of virus to the first we..." Tag: Visual edit

13 September 2021

• 13:4213:42, 13 September 2021 diff hist +321‎ Exit Protocol ‎No edit summary Tag: Visual edit