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Combined display of all available logs of Heit Lab Wiki. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 18:52, 17 January 2025 Akipp4 talk contribs created page Restriction Digest (Created page with " Protocol 1. Check the NEB (or other supplier) website to confirm enzyme compatibility 2. In a PCR tube add the reaction components according to the table listed below 3. Incubate at 37°C for 30 min if time-saver qualified, 60 minutes if not Example Reaction Component Volume ddH2O Up to 50 µL 10X rCutSmart Buffer 5 µL DNA x (1 µg) Enzyme 1 1 µL Enzyme 2 1 µL Notes - NEB High-Fidelity (HF) enzymes are ordered whenever possible, they all use rCutSmart reactio...")
- 17:16, 11 January 2022 Akipp4 talk contribs created page Transduction of THP-1s (Created page with "'''Transduction Reaction''' * The volume of the reaction is based on the amount of virus available, the number of cells and the MOI being used * Prepare the mixture as follows: ** X uL of concentrated virus ** X uL of SF Macrophage Media (chose this volume based on the vessel the cells will be grown in ** 1000X polybrene '''Protocol''' # Count cells, add cells to appropriate size tube, spin down at 400 x G for 5 minutes # Re-suspend cells in the transduct...") Tag: Visual edit
- 15:17, 25 November 2021 Akipp4 talk contribs created page Titering Pseudo-typed Lentiviruses (Created page with "- for titering one “batch” of collected virus '''Cell Seeding''' 1. Seed 6 wells of a 12 well plate with 30 000 – 50 000 HeLas per well on coverslips '''Transduction''' 1. Prepare “transduction media” by mixing 6 µL of polybrene (1000X) in 6 mL of DMEM+10% FBS 2. Remove media from the plate and replace with 900 µL of transduction media in each well 3. Begin the serial dilution by adding 100 µL of virus to the first we...") Tag: Visual edit