Titering Pseudo-typed Lentiviruses: Revision history

Diff selection: Mark the radio buttons of the revisions to compare and hit enter or the button at the bottom.
Legend: (cur) = difference with latest revision, (prev) = difference with preceding revision, m = minor edit.

curprev 19:2219:22, 29 November 2021‎ Akipp4 talk contribs‎ 3,043 bytes 0‎ No edit summary Tag: Visual edit

curprev 15:1715:17, 25 November 2021‎ Akipp4 talk contribs‎ 3,043 bytes +3,043‎ Created page with "- for titering one “batch” of collected virus '''Cell Seeding''' 1. Seed 6 wells of a 12 well plate with 30 000 – 50 000 HeLas per well on coverslips '''Transduction''' 1. Prepare “transduction media” by mixing 6 µL of polybrene (1000X) in 6 mL of DMEM+10% FBS 2. Remove media from the plate and replace with 900 µL of transduction media in each well 3. Begin the serial dilution by adding 100 µL of virus to the first we..." Tag: Visual edit

Titering Pseudo-typed Lentiviruses: Revision history

6 December 2021

29 November 2021

25 November 2021

Navigation menu