Transduction of THP-1s: Revision history

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11 January 2022

  • curprev 17:1617:16, 11 January 2022‎ Akipp4 talk contribs‎ 1,238 bytes +1,238‎ Created page with "'''Transduction Reaction''' * The volume of the reaction is based on the amount of virus available, the number of cells and the MOI being used   *   Prepare the mixture as follows: **  X uL of concentrated virus **  X uL of SF Macrophage Media (chose this volume based on the vessel the cells will be grown in ** 1000X polybrene '''Protocol''' # Count cells, add cells to appropriate size tube, spin down at 400 x G for 5 minutes # Re-suspend cells in the transduct..." Tag: Visual edit
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