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• 17:16, 11 January 2022 Akipp4 talk contribs created page Transduction of THP-1s (Created page with "'''Transduction Reaction''' * The volume of the reaction is based on the amount of virus available, the number of cells and the MOI being used   *   Prepare the mixture as follows: **  X uL of concentrated virus **  X uL of SF Macrophage Media (chose this volume based on the vessel the cells will be grown in ** 1000X polybrene '''Protocol''' # Count cells, add cells to appropriate size tube, spin down at 400 x G for 5 minutes # Re-suspend cells in the transduct...") Tag: Visual edit