User contributions for Admin

2 March 2022

• 17:3517:35, 2 March 2022 diff hist +10‎ N File:50mm Align.png ‎No edit summary current
• 17:0317:03, 2 March 2022 diff hist +2,121‎ N 3D Printed Microscopy Chambers ‎ Created page with "Customized imaging chambers can be 3D printed using FDM or SLA printing technology, and attached to a #1.5 thickness coverglass to allow for high-resolution imaging. When prepared properly, these chambers show no cytotoxicity or other signs of altered cell behaviour, even in cells cultured for multiple days. These are the chamber designs used in the [https://www.phagocytes.ca Heit Lab], and are based on our publication in Biochemistry and Cell Biology<ref name=":0">Teppe..." Tag: Visual edit
• 17:0317:03, 2 March 2022 diff hist +10‎ N File:18mm 4well.png ‎No edit summary current
• 17:0217:02, 2 March 2022 diff hist +10‎ N File:18mm 2well.png ‎No edit summary current
• 17:0217:02, 2 March 2022 diff hist +10‎ N File:18mm 1well.png ‎No edit summary current
• 17:0117:01, 2 March 2022 diff hist +11‎ N File:50mm 15well.png ‎No edit summary current
• 17:0117:01, 2 March 2022 diff hist +11‎ N File:50mm 12well.png ‎No edit summary current
• 17:0017:00, 2 March 2022 diff hist +11‎ N File:50mm 10well.png ‎No edit summary current
• 17:0017:00, 2 March 2022 diff hist +10‎ N File:50mm 9well.png ‎No edit summary current
• 17:0017:00, 2 March 2022 diff hist +10‎ N File:50mm 8well.png ‎No edit summary current
• 16:5916:59, 2 March 2022 diff hist +15‎ N File:50mm 6well Long.png ‎No edit summary current
• 16:5916:59, 2 March 2022 diff hist +10‎ N File:50mm 6well.png ‎No edit summary current
• 16:5816:58, 2 March 2022 diff hist +15‎ N File:50mm 4well Long.png ‎No edit summary current
• 16:5816:58, 2 March 2022 diff hist +10‎ N File:50mm 4well.png ‎No edit summary current
• 16:5716:57, 2 March 2022 diff hist +15‎ N File:50mm 3well Long.png ‎No edit summary current
• 16:5716:57, 2 March 2022 diff hist +10‎ N File:50mm 3well.png ‎No edit summary current
• 16:5716:57, 2 March 2022 diff hist +15‎ N File:50mm 2well Long.png ‎No edit summary current
• 16:5616:56, 2 March 2022 diff hist +10‎ N File:50mm 2well.png ‎No edit summary current
• 16:5516:55, 2 March 2022 diff hist +10‎ N File:50mm 1well.png ‎No edit summary current
• 16:4616:46, 2 March 2022 diff hist +36‎ m Main Page ‎No edit summary Tag: Visual edit
• 13:4813:48, 2 March 2022 diff hist −317‎ m Immuno-FISH ‎No edit summary current Tag: Visual edit
• 13:0413:04, 2 March 2022 diff hist +371‎ m Immunostaining ‎No edit summary Tag: Visual edit
• 12:4312:43, 2 March 2022 diff hist +332‎ m Immuno-FISH ‎No edit summary Tag: Visual edit

26 January 2022

• 13:1913:19, 26 January 2022 diff hist +8‎ m Immuno-FISH ‎ →‎General: Tag: Visual edit

20 January 2022

• 18:2818:28, 20 January 2022 diff hist +8‎ m Immuno-FISH ‎ →‎General: Tag: Visual edit

18 January 2022

• 17:1317:13, 18 January 2022 diff hist +2,501‎ N Nucleofector ‎ Created page with "Protocols and settings for the nucleofector. ==Reusing Cuvettes== Nucleofector cuvettes can be reused several times, allowing for far more transfections to be conducted than the kits claim. Reuse is limited to 5-8 transfections, and it is a good idea to try and use the same cuvette for the same plasmids to limit the risk of cross-contamination. Decreasing transfection efficiency, or sparking during electroporation, are signs that the cuvette is no longer usable. ===Cle..." current Tag: Visual edit
• 16:4116:41, 18 January 2022 diff hist +18‎ m Main Page ‎No edit summary Tag: Visual edit

11 January 2022

• 16:4216:42, 11 January 2022 diff hist +47‎ m Immuno-FISH ‎No edit summary Tag: Visual edit

25 November 2021

• 14:1814:18, 25 November 2021 diff hist −54‎ m Nitrogen Cavitation ‎ →‎Procedure current Tag: Visual edit

18 November 2021

• 19:0919:09, 18 November 2021 diff hist −61‎ m Immuno-FISH ‎ →‎General: Tag: Visual edit
• 19:0719:07, 18 November 2021 diff hist +1,431‎ m Immuno-FISH ‎No edit summary Tag: Visual edit
• 18:5018:50, 18 November 2021 diff hist +1,978‎ N Immuno-FISH ‎ Created page with "This is a protocol for performing Fluorescence In-Situ Hybridization alongside immunostained cells. The protocol assumes that the cells are already grown on a coverslip, treat..." Tag: Visual edit
• 18:0918:09, 18 November 2021 diff hist +17‎ m Main Page ‎No edit summary Tag: Visual edit
• 16:0716:07, 18 November 2021 diff hist −6,333‎ FRET in FIJI ‎ →‎UDC-FRET current Tag: Visual edit

17 November 2021

• 18:3918:39, 17 November 2021 diff hist 0‎ m FRET in FIJI ‎ →‎Acquisition and Calculating FRET Tag: Visual edit
• 18:3218:32, 17 November 2021 diff hist +3,147‎ m FRET in FIJI ‎ →‎UDC-FRET Tag: Visual edit
• 18:3118:31, 17 November 2021 diff hist +711‎ m FRET in FIJI ‎ →‎Acquisition and Calculating FRET Tag: Visual edit
• 18:2818:28, 17 November 2021 diff hist +43‎ m FRET in FIJI ‎ →‎Method 2 - Corrected Sensitized Emission Tag: Visual edit
• 18:2618:26, 17 November 2021 diff hist +54‎ N File:FRET equasion.png ‎No edit summary current
• 18:2118:21, 17 November 2021 diff hist +10,806‎ N FRET in FIJI ‎ Created page with "There are two methods that can be easily implemented in FIJI and on our microscope to calculate FRET ratios in fixed or live cell images. The methods, and FIJI scripts to auto..." Tag: Visual edit
• 15:5915:59, 17 November 2021 diff hist +32‎ m Main Page ‎No edit summary Tag: Visual edit
• 15:5815:58, 17 November 2021 diff hist +68‎ Live Cell FRET ‎No edit summary current Tag: Visual edit

16 November 2021

• 14:4014:40, 16 November 2021 diff hist +17‎ m Nitrogen Cavitation ‎No edit summary Tag: Visual edit
• 14:4014:40, 16 November 2021 diff hist +309‎ m Nitrogen Cavitation ‎ →‎Protocol 1 - Phagosomes/Efferosomes Tag: Visual edit
• 14:3514:35, 16 November 2021 diff hist −14‎ m Nitrogen Cavitation ‎ →‎Lysis Buffer Tag: Visual edit
• 14:3414:34, 16 November 2021 diff hist +4,060‎ N Nitrogen Cavitation ‎ Created page with "Nitrogen caviatation is a gentle way of lysing cells, and can be used to recover intact organelles, efferosomes, phagosomes, etc. We have two protocols, optimized for differen..." Tag: Visual edit
• 14:0414:04, 16 November 2021 diff hist +25‎ m Main Page ‎No edit summary Tag: Visual edit

15 November 2021

• 14:4614:46, 15 November 2021 diff hist +15‎ m Western Blotting ‎ →‎10x Running Buffer current Tag: Visual edit

18 February 2021

• 12:5212:52, 18 February 2021 diff hist +71‎ m Main Page ‎No edit summary Tag: Visual edit

1 February 2021

• 20:1720:17, 1 February 2021 diff hist +23‎ m Main Page ‎No edit summary Tag: Visual edit