3D Printed Microscopy Chambers
Customized imaging chambers can be 3D printed using FDM or SLA printing technology, and attached to a #1.5 thickness coverglass to allow for high-resolution imaging. When prepared properly, these chambers show no cytotoxicity or other signs of altered cell behaviour, even in cells cultured for multiple days. These are the chamber designs used in the Heit Lab, and are based on our publication in Biochemistry and Cell Biology[1], and on the Mod3D preprint[2] by the Truant Lab.
In addition to directly printing chambers, 3D printing can also be used to make moulds for casting PDMS chambers, as described in our publication in Biochemistry and Cell Biology[1].
Chamber Designs
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- ↑ 1.0 1.1 Tepperman A, Zheng DJ, Taka MA, Vrieze A, Le Lam A, Heit B. Customizable live-cell imaging chambers for multimodal and multiplex fluorescence microscopy. Biochem Cell Biol. 2020 Oct;98(5):612-623. doi: 10.1139/bcb-2020-0064. Epub 2020 Apr 27. PMID: 32339465. Link to Paper. Link to Preprint.
- ↑ C. Barba Bazan, S. Goss, C. Peng, N. Begeja, CE. Suart, K. Neuman, Ray Truant. Mod3D: A Low-Cost, Flexible Modular System of Live-Cell Microscopy Chambers and Holders. bioRxiv 2021.10.18.462400; doi: https://doi.org/10.1101/2021.10.18.462400