All public logs

Combined display of all available logs of Heit Lab Wiki. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).

• 15:17, 25 November 2021 Akipp4 talk contribs created page Titering Pseudo-typed Lentiviruses (Created page with "-       for titering one “batch” of collected virus '''Cell Seeding''' 1.     Seed 6 wells of a 12 well plate with 30 000 – 50 000 HeLas per well on coverslips '''Transduction''' 1.     Prepare “transduction media” by mixing 6 µL of polybrene (1000X) in 6 mL of DMEM+10% FBS 2.     Remove media from the plate and replace with 900 µL of transduction media in each well 3.     Begin the serial dilution by adding 100 µL of virus to the first we...") Tag: Visual edit