Antibiotics: Difference between revisions
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(Created page with "== Protocol == ==== Ampicillin<br> ==== Preparation of 5 mL of 100 mg/mL: #Add 0.5 g of ampicilin into a 12 mL tube. #Add 2.5 mL of ddH<sub>2</sub>O into the tube and mix g...") |
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Preparation of 5 mL of 100 mg/mL: | Preparation of 5 mL of 100 mg/mL: | ||
#Add 0.5 g of | #Add 0.5 g of ampicillin into a 12 mL tube. | ||
#Add 2.5 mL of ddH<sub>2</sub>O into the tube and mix gently by tapping the side of the tube. | #Add 2.5 mL of ddH<sub>2</sub>O into the tube and mix gently by tapping the side of the tube. | ||
#Add 2.5 mL of 100% ethanol and mix the tube again. | #Add 2.5 mL of 100% ethanol and mix the tube again. | ||
#Filter with a 0.22 μm syringe filter | |||
#Aliquot out 1 mL into each of 5 microcentrifuge tubes. | #Aliquot out 1 mL into each of 5 microcentrifuge tubes. | ||
#Label the tubes as 1000x ampicillin and write the date. | #Label the tubes as 1000x ampicillin and write the date. | ||
#Place tubes in the antibiotics box in the -20 °C freezer.<br> | #Place tubes in the antibiotics box in the -20 °C freezer.<br> | ||
Latest revision as of 15:20, 1 March 2022
Protocol
Ampicillin
Preparation of 5 mL of 100 mg/mL:
- Add 0.5 g of ampicillin into a 12 mL tube.
- Add 2.5 mL of ddH2O into the tube and mix gently by tapping the side of the tube.
- Add 2.5 mL of 100% ethanol and mix the tube again.
- Filter with a 0.22 μm syringe filter
- Aliquot out 1 mL into each of 5 microcentrifuge tubes.
- Label the tubes as 1000x ampicillin and write the date.
- Place tubes in the antibiotics box in the -20 °C freezer.
Note: Ampicillin stock will only be good for approximately 6 months.
Kanamycin
Preparation of 5 mL of 50 mg/mL
- Add 0.25 g of kanamycin into a 12 mL tube.
- Add 5 mL ddH2O into the tube and mix by shaking tube.
- Aliquot out 1 mL into each of 5 microcentrifuge tubes.
- Label the tubes as 1000x kanamycin and write the date.
- Place the tubes in the antibiotics box in the -20 °C freezer.
Note: Kanamycin tubes must be kept in the fridge after being removed from the freezer.