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*[[Saving Experimental Files|Proper Saving of Experimental Files]] | *[[Saving Experimental Files|Proper Saving of Experimental Files]] | ||
*[[Setting Up Network Drives|Setting up Access to Network Drives & Wiki]] | *[[Setting Up Network Drives|Setting up Access to Network Drives & Wiki]] | ||
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==== DNA/Cloning ==== | ==== DNA/Cloning ==== | ||
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*[[Ligation]] | *[[Ligation]] | ||
*[[PCR]] | *[[PCR]] | ||
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|'''Transfections and Transductions''' | |'''Transfections and Transductions''' | ||
* [[J774 Cell Transfection]] | * [[J774 Cell Transfection]] | ||
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*[[Freezing and Thawing Cells]] | *[[Freezing and Thawing Cells]] | ||
*[[Water Bath Antibiotic Solution]] | *[[Water Bath Antibiotic Solution]] | ||
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==== Cell Biology ==== | ==== Cell Biology ==== | ||
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*[[Primary Human Macrophages]] | *[[Primary Human Macrophages]] | ||
*[[Primary Macrophage Culture|Primary Murine Macrophages]] | *[[Primary Macrophage Culture|Primary Murine Macrophages]] | ||
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==== Lipids ==== | ==== Lipids ==== | ||
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*[[Phagosome Isolation]] | *[[Phagosome Isolation]] | ||
*[[Synchronised Phagocytosis]] | *[[Synchronised Phagocytosis]] | ||
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==== Protein Work ==== | ==== Protein Work ==== | ||
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*[[Updated FPLC Size Exclusion Procedure]] | *[[Updated FPLC Size Exclusion Procedure]] | ||
*[[Western Blotting]] | *[[Western Blotting]] | ||
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|'''Microscopy''' | |'''Microscopy''' | ||
*[[Nucleofector|3D Printed PDMS Chambers]] | *[[Nucleofector|3D Printed PDMS Chambers]] | ||
Revision as of 20:15, 23 February 2022
Welcome to the protocol wiki for the lab of Dr. Bryan Heit, at the University of Western Ontario. This site contains the protocols, and other information, we frequently use in our lab. Only laboratory members may edit pages, but anyone is welcome to use these protocols. If you use these protocol, please cite us. A link for generating citations can be found on the left side of the page.
Consult the User's Guide for information on using the wiki software.
Lab members:
- To get a wiki account please contact Dr. Heit.
- Log in (upper-right side of screen) to add/edit pages.
- Please follow these formatting instructions when editing/creating protocols.
- To create a new page, follow these instructions.
Index of Protocols
Links to More Protocols
General Protocol Sites
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Free Science Ebooks
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Useful Links
Molecular Biology Databases
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Molecular Biology Tools
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Microscopy Tools
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Journal Resources
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In Vivo Tools
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Protease Tools |
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Genomics Resources |
Chemical Tools
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Lipid Tools
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