Main Page: Difference between revisions
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*[[Saving Experimental Files|Proper Saving of Experimental Files]] | *[[Saving Experimental Files|Proper Saving of Experimental Files]] | ||
*[[Setting Up Network Drives|Setting up Access to Network Drives & Wiki]] | *[[Setting Up Network Drives|Setting up Access to Network Drives & Wiki]] | ||
| | | | ||
==== DNA/Cloning ==== | ==== DNA/Cloning ==== | ||
*[[Agarose Gels]] | |||
*[[Colony PCR]] | *[[Colony PCR]] | ||
*[[Gibson Assembly]] | *[[Gibson Assembly]] | ||
*[[Ligation]] | *[[Ligation]] | ||
*[[PCR]] | *[[PCR]] | ||
*[[Restriction Digest]] | |||
| | | | ||
|'''Transfections and Transductions''' | |'''Transfections and Transductions''' | ||
* [[J774 Cell Transfection]] | * [[J774 Cell Transfection]] | ||
* [[Raw Cell Transfection]] | * [[Raw Cell Transfection]] | ||
* [[THP1 Culture and Differentiation]] | |||
* [[Neon® Transfection System]] | * [[Neon® Transfection System]] | ||
* [[Titering Pseudo-typed Lentiviruses]] | * [[Titering Pseudo-typed Lentiviruses]] | ||
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|- style="vertical-align:top;" | |- style="vertical-align:top;" | ||
| | | | ||
==== General Protocols ==== | ==== General Protocols ==== | ||
*[[Antibiotics]] | *[[Antibiotics]] | ||
*[[Antibiotic Plates]] | *[[Antibiotic Plates]] | ||
| Line 54: | Line 53: | ||
*[[Freezing and Thawing Cells]] | *[[Freezing and Thawing Cells]] | ||
*[[Water Bath Antibiotic Solution]] | *[[Water Bath Antibiotic Solution]] | ||
| | | | ||
==== Cell Biology ==== | ==== Cell Biology ==== | ||
| Line 96: | Line 91: | ||
*[[Phagosome Isolation]] | *[[Phagosome Isolation]] | ||
*[[Synchronised Phagocytosis]] | *[[Synchronised Phagocytosis]] | ||
| | | | ||
==== Protein Work ==== | ==== Protein Work ==== | ||
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| | | | ||
|'''Microscopy''' | |'''Microscopy''' | ||
*[[ | *[[3D Printed PDMS Chambers]] | ||
*[[ | *[[Acid Washing Coverslips]] | ||
*[[ | *[[Competition of Charged Molecules with Lipophilic Cations]] | ||
*[[ | *[[Immunostaining|Fluorescent Immunostaining]] | ||
*[[ | *[[FRET in FIJI]] | ||
*[[ | *[[Inhibition of Focal Contact Signaling]] | ||
*[[ | *[[Immuno-FISH]] | ||
*[[ | *[[Live Cell FRET]] [[Nucleofector|(depreciated)]] | ||
*[[ | *[[Reducing Photobleaching]] | ||
*[[ | *[[Single Particle Tracking]] | ||
*[[ | *[[Staining for GSD]] | ||
*[[ | *[[Traction Force Microscopy]] | ||
*[[3D Printed Microscopy Chambers]] | |||
* | *[[Micropatterning Proteins]] | ||
*[[Frustrated Phagocytosis]] | |||
|} | |} | ||
Latest revision as of 18:50, 17 January 2025
Welcome to the protocol wiki for the lab of Dr. Bryan Heit, at the University of Western Ontario. This site contains the protocols, and other information, we frequently use in our lab. Only laboratory members may edit pages, but anyone is welcome to use these protocols. If you use these protocol, please cite us. A link for generating citations can be found on the left side of the page.
Consult the User's Guide for information on using the wiki software.
Lab members:
- To get a wiki account please contact Dr. Heit.
- Log in (upper-right side of screen) to add/edit pages.
- Please follow these formatting instructions when editing/creating protocols.
- To create a new page, follow these instructions.
Index of Protocols
Lab Operations |
DNA/Cloning |
Transfections and Transductions | ||
General Protocols |
Cell Biology |
LipidsBead Preparation |
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Bacteria Work
Phagocytosis Protocols |
Protein Work |
Microscopy
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Links to More Protocols
General Protocol Sites
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Free Science Ebooks
|
Useful Links
Molecular Biology Databases
|
Molecular Biology Tools
|
Microscopy Tools
|
|
Journal Resources
|
In Vivo Tools
|
Protease Tools |
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Genomics Resources |
Chemical Tools
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Lipid Tools
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