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*[[Ligation]] | *[[Ligation]] | ||
*[[Gibson Assembly]] | *[[Gibson Assembly]] | ||
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==== Microscopy ==== | ==== Microscopy ==== | ||
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==== Lipids ==== | ==== Lipids ==== | ||
*[[Asymmetric liposomes]] | *[[Asymmetric liposomes]] | ||
*[[Lipid Extraction from Cells]] | *[[Lipid Extraction from Cells]] | ||
'''Bead Preparation''' | |||
* [[Opsonization]] | |||
*[[Preparation of Silica-Magnetic Beads]] | |||
*[[Lipisome and Lipid-Coated Beads]] | *[[Lipisome and Lipid-Coated Beads]] | ||
*[[Lipid Coated Bead Preparation]] | |||
| | | | ||
|- style="vertical-align:top;" | |- style="vertical-align:top;" | ||
| | |'''Bacteria Work''' | ||
*[[Competent e coli|Generating Competent ''E. coli'' (TFB, BL21 & ZYCY10P3S2T)]] | |||
*[[Generating Minicircles]] | |||
*[[Inside-out Labelling of Bacteria]] | |||
*[[Labelled E coli]] | |||
*[[Preparation of Digestion-Tracking Bacteria]] | |||
*[[Quick 'n' Easy Competent E. coli|Quick 'n' Easy Competent ''E. coli'' (Dh5a)]] | |||
*[[E. coli Transduction|Transducing ''E. coli'']] | |||
*[[Transformation]] | |||
==== Phagocytosis Protocols ==== | ==== Phagocytosis Protocols ==== | ||
*[[ | *[[Gentamicin Protection Assay]] | ||
*[[Phagosome Isolation]] | *[[Phagosome Isolation]] | ||
| | | | ||
==== Cell Biology ==== | ==== Cell Biology ==== | ||
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*[[Apoptosis Detection with AnnexinV and PI]] | *[[Apoptosis Detection with AnnexinV and PI]] | ||
*[[Immuno-FISH]] | *[[Immuno-FISH]] | ||
*[[Primary Human Macrophages]] | |||
*[[Synchronised Phagocytosis]] | |||
|'''Transfections and Transductions''' | |'''Transfections and Transductions''' | ||
* [[J774 Cell Transfection]] | * [[J774 Cell Transfection]] |
Revision as of 16:20, 17 January 2022
Welcome to the protocol wiki for the lab of Dr. Bryan Heit, at the University of Western Ontario. This site contains the protocols, and other information, we frequently use in our lab. Only laboratory members may edit pages, but anyone is welcome to use these protocols. If you use these protocol, please cite us. A link for generating citations can be found on the left side of the page.
Consult the User's Guide for information on using the wiki software.
Lab members:
- To get a wiki account please contact Dr. Heit.
- Log in (upper-right side of screen) to add/edit pages.
- Please follow these formatting instructions when editing/creating protocols.
- To create a new page, follow these instructions.
Index of Protocols
Lab Operations |
DNA/Cloning |
Microscopy
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General Protocols |
Protein work |
Lipids
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Bacteria Work
Phagocytosis Protocols |
Cell Biology |
Transfections and Transductions
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Links to More Protocols
General Protocol Sites
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Free Science Ebooks
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Useful Links
Molecular Biology Databases
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Molecular Biology Tools
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Microscopy Tools
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Journal Resources
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In Vivo Tools
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Protease Tools |
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Genomics Resources |
Chemical Tools
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Lipid Tools
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